Recently Published

The following are studies that validate the Orthotopic PDX technique and have been published in peer-reviewed journals

Featured Study

  1. Eckardt M.D., Russell T.A., Murakami T., Singh A.S., Kiyuna T., Igarashi K., Kawaguchi K., Li Y., Crompton J., Dry S.M., Federman N., Chmielowski B., Shurrell E., Hoffman R.M., Eilber F.C.  Clinical factors that affect the establishment of soft tissue sarcoma patient-derived orthotopic xenografts: A UCLA sarcoma program prospective clinical trial. JCO Precision Oncology, 2017. | Download pdf |

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Here is a selection of recently published peer-reviewed studies (2016 and 2017) that demonstrate the efficacy of the Orthotopic PDX technique. 

  1. Igarashi, K., Kawaguchi, A., Murakami, T., Kiyuna, T., Miyake, K., Nelson, S.D., Dryd, S.M., Lid, Y., Yanagawae, J., Russell, T.A., Singh, A.S., Yamamotoc, N., Hayashic, K., Kimurac, H., Miwac, S., Tsuchiyac, H., Eilber, F.C., Hoffman, R.M. Intra-arterial administration of tumor-targeting Salmonella typhimurium A1-R regresses a cisplatin-resistant relapsed osteosarcoma in a patient-derived orthotopic xenograft (PDOX) mouse model. Cell Cycle, 2017, Vol. 16, No. 12, 1164–1170 https://doi.org/10.1080/15384101.2017.1317417. | Download pdf |
  2. Igarashi, K., Kawaguchi, K., Kiyuna, T., Murakami, T., Miwa, S., Nelson, S.D., Dry, S.M., Lid, Y., Singh, A., Kimura, H., Hayashi, K., Yamamoto, N., Tsuchiya, H., Eilber, F.C., Hoffman, R.M. Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model. Cell Cycle, 2017, Vol. 16, No. 1, 91–94 http://dx.doi.org/10.1080/15384101.2016.1252885. | Download pdf |
  3. Igarashi, K., Kawaguchi, A., Murakami, T., Kiyuna, T., Miyake, K., Singh, A.S., Nelson, S.D., Dry, S.M., Li, Y., Yamamoto, N., Hayashi, K., Kimura, H., Miwa, S., Tsuchiya, H., Eilber, F.C., Hoffman, R.H. High efficacy of pazopanib on an undifferentiated spindle-cell sarcoma resistant to first-line therapy is identified with a patient-derived orthotopic xenograft (PDOX) nude mouse model.  J. Cell. Biochem. 118, 2739-3743, 2017. | Download pdf |
  4. Kawaguchi, K., Igarahi, K., Murakami, T., Chmielowski, B., Kiyuna, T., Zhao, M., Zhang, Y., Singh, A., Unno, M., Nelson, S.D., Russell, T.A., Dry, S.M., Eilber, F.C., Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R combined with temozolomide regresses malignant melanoma with a BRAF-V600E mutation in a patient-derived orthotopic xenograft (PDOX) model. Oncotarget, 2016, Vol. 7, (No. 52), pp: 85929-85936. | Download pdf |
  5. Kawaguchi, K., Murakami, T., Chmielowski, B., Igarashi, K., Kiyuna, T., Unno, M., Nelson, S.D., Russell, T.A., Dry, S.M., Li, Y., Eilber, F.C., Hoffman, R.M. Vemurafenib-resistant BRAF-V600E-mutated melanoma is regressed by MEK-targeting drug trametinib, but not cobimetinib in a patient-derived orthotopic xenograft (PDOX) mouse model. Oncotarget, Vol. 7, No. 44. | Download pdf |
  6. Kawaguchi, K., Igarashi, K., Murakami, T., Zhao, M., Zhang, Y., Chmielowski, B., Kiyuna, T., Nelson, S.D., Russell, T.A., Dry, S.M., Li, Y., Unno, M., Eilber, F.C., Hoffman, R.M. Tumor-Targeting Salmonella typhimurium A1-R Sensitizes Melanoma With a BRAF-V600E Mutation to Vemurafenib in a Patient-Derived Orthotopic Xenograft (PDOX) Nude Mouse Model. Journal of Cellular Biochemistry 118:2314–2319 (2017). | Download pdf |
  7. Kawaguchi, K., Igarash, K., Murakami, T., Kiyuna, T., Nelson, S.D., Dry, S.M., Li, Y., Russell, T.A., Singh, A.S., Chmielowski, B., Unno, M., Eilber, F.C., Hoffman, R.M. Combination of gemcitabine and docetaxel regresses both gastric leiomyosarcoma proliferation and invasion in an imageable patient-derived orthotopic xenograft (iPDOX) model.  Cell Cycle 16, 1063-1069, 2017.  | Download pdf |
  8. Kiyuna, T., Murakami, T., Tome, Y., Igarashi, K., Kawaguchi, K., Russell, T., Eckardt, M.A., Crompton, J., Singh, A., Bernthal, N., Bukata, S., Federman, N., Kanaya, F., Eilber, F.C., Hoffman, R.M. Labeling the Stroma of a Patient-Derived Orthotopic Xenograft (PDOX) Mouse Model of Undifferentiated Pleomorphic Soft-Tissue Sarcoma With Red Fluorescent Protein for Rapid Non-Invasive Imaging for Drug Screening. Journal of Cellular Biochemistry 118:361–365 (2017). | Download pdf |
  9. Murakami, T., Singh, A.S., Kiyuna, T., Dry, S.M., Li, Y., James, A.W., Igarashi, K., Kawaguchi, K., DeLong, J.C., Zhang, Y., Hiroshima, Y., Russell, T., Eckardt, M.A., Yanagawa, J., Federman, N., Matsuyama, R., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., Eilberg, F.C., Hoffman, R.M. Effective molecular targeting ofCDK4/6 and IGF-1R in a rare FUS-ERG fusion CDKN2A-deletion doxorubicin-resistant Ewing’s sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model.  Oncotarget 7, 47556-47564, 2016. | Download pdf |
  10. Murakami, T., Igarashi, K., Kawaguchi, K., Kiyuna, T., Zhan, Y., Zhao, M., Hiroshima, Y., Nelson, S.D., Dry, S.M., Li, Y., Yanagawa, J., Russell, T. Federman, N., Singh, A., Irmina Elliott, I., Matsuyama, R., Chishima, T., Tanaka, K., Endo, I., Eilber, F.C., Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R regresses an osteosarcoma in a patient-derived xenograft model resistant to a molecular-targeting drug. Oncotarget, 2017, Vol. 8, (No. 5), pp: 8035-8042. | Download pdf |
  11. Murakami, T., Li, S., Han, Q., Tan, Y., Kiyuna, T., Igarashi, K., Kawaguchi, K., Hwang, H.K., Miyake, K., Singh, A.S., Nelson, S.D., Dry, S.M., Li, Y., Hiroshima, Y., Lwin, T.M., DeLong, J.C., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., Eilber, F.C., Hoffman, R.M. Recombinant methioninase effectively targets a Ewing’s sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model. Oncotarget, 2017, Vol. 8, (No. 22), pp: 35630-35638. | Download pdf |
  12. Igarashi, K., Kawaguchi, K., Kiyuna, T., Murakami, T., Miwa, S., Nelson, S.D., Dry, S.M., Li, Y., Singh, A., Kimura, H., Hayashi, K., Yamamoto, N., Tsuchiya, H., Eilber, F.C., Hoffman, R.M.  Patient-derived orthotopic xenograft (PDOX) mouse model of adult rhabdomyosarcoma invades and recurs after resection in contrast to the subcutaneous ectopic model.  Cell Cycle 16, 91-94, 2017. | Download pdf |
  13. Murakami, T., DeLong, J., Eilber, F.C., Zhao, M., Zhang, Y., Zhang, N., Singh, A., Russell, T., Deng, S., Reynoso, J., Quan, C., Hiroshima, Y., Matsuyama, R., Chishima, T., Tanaka, K., Bouvet, M., Chawla, S., Endo, I., and Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R in combination with doxorubicin eradicate soft tissue sarcoma in a patient-derived orthotopic xenograft PDOX model. Oncotarget 7, 12783-12790, 2016. | Download pdf |
  14. Kiyuna, T., Murakami, T., Tome, Y., Kawaguchi, K., Igarashi, K., Zhang, Y, Zhao, M., Li, Y., Bouvet, M., Kanaya, F., Singh, A., Dry, S., Eilber, F.C., and Hoffman, R.M. High efficacy of tumor-targeting Salmonella typhimurium A1-R on a doxorubicin- and dactolisib-resistant follicular dendritic-cell sarcoma in a patient-derived orthotopic xenograft PDOX nude mouse model. Oncotarget 7, 33046-33054, 2016. | Download pdf |
  15. Yamamoto, M., Zhao, M., Hiroshima, Y., Zhang, Y., Shurell, E., Eilber, F.C., Bouvet, M., Noda, M., Hoffman, R.M. Efficacy of tumor-targeting Salmonella typhimurium A1-R on a melanoma patient-derived orthotopic xenograft (PDOX) nude-mouse model. PLoS One 11, e0160882, 2016. | Download pdf |
  16. Murakami, T., Singh, A.S., Kiyuna, T., Dry, S.M., Li, Y., James, A.W., Igarashi, K., Kawaguchi, K., DeLong, J.C., Zhang, Y., Hiroshima, Y., Russell, T., Eckardt, M.A., Yanagawa, J., Federman, N., Matsuyama, R., Chishima, T., Tanaka, K., Bouvet, M., Endo, I., Eilber, F.C., and Hoffman, R.M. Effective molecular targeting of CDK4/6 and IGF-1R in a rare FUS-ERG fusion CDKN2A-deletion doxorubicin-resistant Ewing’s sarcoma in a patient-derived orthotopic xenograft (PDOX) nude-mouse model.Oncotarget, Epub ahead of print. | Download pdf |
  17. Kiyuna, T., Murakami, T., Tome, Y., Igarashi, K., Kawaguchi, K., Russell, T., Eckhardt, M.A., Crompton, J., Singh, A., Bernthal, N., Bukata, S., Federman, N., Kanaya, F., Eilber, F.C., and Hoffman, R.M. Labeling the stroma of a patient-derived orthotopic xenograft (PDOX) mouse models of undifferentiated pleomorphic soft-tissue sarcoma with red fluorescent protein for rapid non-invasive drug screening. J. Cell. Biochem., Epub ahead of print. | Download pdf |
  18. Kawaguchi, K., Murakami, T., Chmiewloski, B., Igarashi, K., Kiyuna, T., Suetsugu, A., Unno, M., Nelson, S.D., Russell, T., Dry, S.M., Li, Y., Eilber, F.C., Hoffman, R.M. Vemurafenib-resistant BRAF-V600E mutated melanoma is regressed by MEK targeting drug trametinib, but not cobimetinib in a patient-derived orthotopic xenograft (PDOX) mouse model. Oncotarget 7, 71737-71743, 2016. | Download pdf |
  19. Kawaguchi, K., Igarashi, K., Murakami, T., Chmiewloski, B., Kiyuna, T., Zhao, M., Zhang, Y., Singh, A., Unno, M., Nelson, S.D., Russell, T., Dry, S.M., Li, Y., Eilber, F.C., Hoffman, R.M. Tumor-targeting Salmonella typhimurium A1-R combined with Temozolomide regresses malignant melanoma with a BRAF-V600 mutation in a patient-derived orthotopic xenograft (PDOX) model. Oncotarget 7, 85929-85936, 2016. | Download pdf |